Positive Immunostaining with Dystrophin Antibodies in mdx Skeletal Muscle.
نویسندگان
چکیده
منابع مشابه
Effect of rapamycin on immunity induced by vector-mediated dystrophin expression in mdx skeletal muscle
Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene. Therapeutic gene replacement of a dystrophin cDNA into dystrophic muscle can provide functional dystrophin protein to the tissue. However, vector-mediated gene transfer is limited by anti-vector and anti-transgene host immunity that causes rejection of the therapeutic protein. We hypothesized that rapamycin (RAPA) ...
متن کاملDystrophin-associated proteins are greatly reduced in skeletal muscle from mdx mice
Dystrophin, the protein product of the human Duchenne muscular dystrophy gene, exists in skeletal muscle as a large oligomeric complex that contains four glycoproteins of 156, 50, 43, and 35 kD and a protein of 59 kD. Here, we investigated the relative abundance of each of the components of the dystrophin-glycoprotein complex in skeletal muscle from normal and mdx mice, which are missing dystro...
متن کاملSuccessful histocompatible myoblast transplantation in dystrophin- deficient mdx mouse despite the production of antibodies against dystrophin
Myoblast transplantation has been considered a potential treatment for some muscular disorders. It has proven very successful, however, only in immunodeficient or immunosuppressed mice. In this study, myoblasts from C57BL10J +/+ mice were transplanted, with no immunosuppressive treatment, in the tibialis anterior of fully histocompatible but dystrophin-deficient C57BL10J mdx/mdx mice. One to 9 ...
متن کاملDystrophin and dystrophin-like proteins in muscle and brain of normal and mdx mice.
tions. Central nervous system (CNS) gp 130 is a major sialylated glycoprotein component of the CNS which is detectable at the synapse. This molecule is, however, heavily glycosylated and is detectable as a component of myelin (P. R. Gordon-Weeks, personal communication). Both immunocytochemical studies and Western blot analysis show that the present antigens are not myelin associated. Furthermo...
متن کاملPurification of dystrophin from skeletal muscle.
Dystrophin was purified from rabbit skeletal muscle by alkaline dissociation of dystrophin-glycoprotein complex which was first prepared by derivatized lectin chromatography. Dystrophin-glycoprotein complex was isolated from digitonin-solubilized rabbit skeletal muscle membranes by a novel two-step method involving succinylated wheat germ agglutinin (sWGA) chromatography and DEAE-cellulose ion ...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 1991
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.67.148